Zero- to Ultralow-Field NMR Spectroscopy of Small Biomolecules

Nuclear magnetic resonance (NMR) spectroscopy is a well-established analytical technique used to study chemicals and their transformations. However, high-field NMR necessitates advanced infrastructure, even cryogen-free benchtop spectrometers cannot be readily assembled from commercially available components. We demonstrate construction of portable zero-field spectrometer employing magnetometer investigate its applications in chemistry. In particular, J-spectra small representative biomolecules [13C]-formic acid, [1-13C]-glycine, [2,3-13C]-fumarate, [1-13C]-d-glucose were acquired, an approach relying on the presence transverse field during detection was investigated for relaxometry purposes. found that water relaxation time strongly depends concentration dissolved d-glucose range 1–10 mM suggesting opportunities indirect assessment glucose aqueous solutions. Extending capabilities solutions simple (amino acids, sugars, metabolites) studies highlights potential noninvasive ZULF sensors outside research laboratories.